random hexamers (New England Biolabs)
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random hexamers
Random Hexamers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hexamer+primer/Random+Primer+6/pmc12875486-304-33-35
Average 94 stars, based on 436 article reviews
Random Hexamers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hexamer+primer/Random+Primer+6/pmc12875486-304-33-35
Average 94 stars, based on 436 article reviews
random hexamers - by Bioz Stars,
2026-09
94/100 stars
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Transfection:Article Title: Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation Article Snippet: .. 48h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M-MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation Article Snippet: .. 48 h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M‐MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Synthesized:Article Title: Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation Article Snippet: .. 48h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M-MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation Article Snippet: .. 48 h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M‐MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Molecular and clinical characteristics related to rhinovirus infection in Brasília, Brazil Article Snippet: .. This primer pair was used to amplify an approximately 540-bp fragment for amplicon sequencing, including part of 5’ UTR and the VP4/VP2 protein gene of HRV A, B, and C. cDNA of the selected samples were synthesized using MMLV transcriptase (Thermo Fisher Scientific, Waltham, USA) with random Reverse Transcription:Article Title: Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation Article Snippet: .. 48h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M-MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Nonsense‐mediated decay factor SMG7 sensitizes cells to TNFα‐induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1 Article Snippet: .. Total RNA was isolated using InviTrap Spin Cell RNA Mini Kit (Stratec, Birkenfeld, Germany), and 500 ng RNA was used for first‐strand cDNA synthesis via random Article Title: Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation Article Snippet: .. 48 h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M‐MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Detection and Molecular Characterization of GI-1 and GI-23 Avian Infectious Bronchitis Virus in Broilers Indicate the Emergence of New Genotypes in Bolivia Article Snippet: .. For cDNA synthesis, 7 μL of total RNA (300 ng) from each sample and 2 μL of random Article Title: Viral Metagenomics Revealed a Novel Cardiovirus in Feces of Wild Rats. Article Snippet: Background/Aims: Cardiovirus is a genus of viruses belonging to the family Picornaviridae.. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China.. Method: Fecal samples were collected from 20 wild rats and pooled into four sample pools and then subjected to libraries construction which were then sequenced on Illumina MiSeq platform. Random Hexamer:Article Title: Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation Article Snippet: .. 48h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M-MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Nonsense‐mediated decay factor SMG7 sensitizes cells to TNFα‐induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1 Article Snippet: .. Total RNA was isolated using InviTrap Spin Cell RNA Mini Kit (Stratec, Birkenfeld, Germany), and 500 ng RNA was used for first‐strand cDNA synthesis via random Article Title: Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation Article Snippet: .. 48 h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M‐MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress Article Snippet: Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. .. In brief, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented and used for cDNA synthesis with random Article Title: Detection and Molecular Characterization of GI-1 and GI-23 Avian Infectious Bronchitis Virus in Broilers Indicate the Emergence of New Genotypes in Bolivia Article Snippet: .. For cDNA synthesis, 7 μL of total RNA (300 ng) from each sample and 2 μL of random Article Title: The regulatory architecture of the primed pluripotent cell state Article Snippet: The eluted sample was further purified using AMPure XP PCR purification beads (Beckman Coulter A63880) at a 1:1 sample to bead ratio, and eluted with 15 μl of nuclease-free water. .. For second-strand synthesis, 1 μl of 10 mM dNTPs and 1 μl of 100 mM adapter-linked random Article Title: Viral Metagenomics Revealed a Novel Cardiovirus in Feces of Wild Rats. Article Snippet: Background/Aims: Cardiovirus is a genus of viruses belonging to the family Picornaviridae.. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China.. Method: Fecal samples were collected from 20 wild rats and pooled into four sample pools and then subjected to libraries construction which were then sequenced on Illumina MiSeq platform. Article Title: Molecular and clinical characteristics related to rhinovirus infection in Brasília, Brazil Article Snippet: .. This primer pair was used to amplify an approximately 540-bp fragment for amplicon sequencing, including part of 5’ UTR and the VP4/VP2 protein gene of HRV A, B, and C. cDNA of the selected samples were synthesized using MMLV transcriptase (Thermo Fisher Scientific, Waltham, USA) with random Amplification:Article Title: Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation Article Snippet: .. 48h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M-MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Disease‐linked TDP‐43 hyperphosphorylation suppresses TDP‐43 condensation and aggregation Article Snippet: .. 48 h after transfection, cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA, M‐MLV reverse transcriptase polymerase (Invitrogen), and 6 μM of random Article Title: Molecular and clinical characteristics related to rhinovirus infection in Brasília, Brazil Article Snippet: .. This primer pair was used to amplify an approximately 540-bp fragment for amplicon sequencing, including part of 5’ UTR and the VP4/VP2 protein gene of HRV A, B, and C. cDNA of the selected samples were synthesized using MMLV transcriptase (Thermo Fisher Scientific, Waltham, USA) with random Isolation:Article Title: Nonsense‐mediated decay factor SMG7 sensitizes cells to TNFα‐induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1 Article Snippet: .. Total RNA was isolated using InviTrap Spin Cell RNA Mini Kit (Stratec, Birkenfeld, Germany), and 500 ng RNA was used for first‐strand cDNA synthesis via random cDNA Synthesis:Article Title: Nonsense‐mediated decay factor SMG7 sensitizes cells to TNFα‐induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1 Article Snippet: .. Total RNA was isolated using InviTrap Spin Cell RNA Mini Kit (Stratec, Birkenfeld, Germany), and 500 ng RNA was used for first‐strand cDNA synthesis via random Article Title: Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress Article Snippet: Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. .. In brief, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented and used for cDNA synthesis with random Article Title: Detection and Molecular Characterization of GI-1 and GI-23 Avian Infectious Bronchitis Virus in Broilers Indicate the Emergence of New Genotypes in Bolivia Article Snippet: .. For cDNA synthesis, 7 μL of total RNA (300 ng) from each sample and 2 μL of random Purification:Article Title: Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress Article Snippet: Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. .. In brief, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented and used for cDNA synthesis with random Article Title: The regulatory architecture of the primed pluripotent cell state Article Snippet: The eluted sample was further purified using AMPure XP PCR purification beads (Beckman Coulter A63880) at a 1:1 sample to bead ratio, and eluted with 15 μl of nuclease-free water. .. For second-strand synthesis, 1 μl of 10 mM dNTPs and 1 μl of 100 mM adapter-linked random Magnetic Beads:Article Title: Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress Article Snippet: Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. .. In brief, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented and used for cDNA synthesis with random Incubation:Article Title: The regulatory architecture of the primed pluripotent cell state Article Snippet: The eluted sample was further purified using AMPure XP PCR purification beads (Beckman Coulter A63880) at a 1:1 sample to bead ratio, and eluted with 15 μl of nuclease-free water. .. For second-strand synthesis, 1 μl of 10 mM dNTPs and 1 μl of 100 mM adapter-linked random DNA Synthesis:Article Title: Viral Metagenomics Revealed a Novel Cardiovirus in Feces of Wild Rats. Article Snippet: Background/Aims: Cardiovirus is a genus of viruses belonging to the family Picornaviridae.. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China.. Method: Fecal samples were collected from 20 wild rats and pooled into four sample pools and then subjected to libraries construction which were then sequenced on Illumina MiSeq platform. Sequencing:Article Title: Molecular and clinical characteristics related to rhinovirus infection in Brasília, Brazil Article Snippet: .. This primer pair was used to amplify an approximately 540-bp fragment for amplicon sequencing, including part of 5’ UTR and the VP4/VP2 protein gene of HRV A, B, and C. cDNA of the selected samples were synthesized using MMLV transcriptase (Thermo Fisher Scientific, Waltham, USA) with random |